TRANSCOBALAMIN-II - COBALAMIN BINDING-SITES ARE PRESENT ON RABBIT GERM-CELLS

被引：6

作者：

BOUKHZER, E

ENNYA, A

FELDEN, F

GERARD, A

NEXO, E

NICOLAS, JP

GERARD, H

GUEANT, JL

机构：

[1] FAC MED VANDOEUVRE NANCY, INSERM, U308, BIOCHIM NUTR LAB, F-54500 VANDOEUVRE LES NANCY, FRANCE

[2] FAC MED VANDOEUVRE NANCY, HISTOL EMBRYOL LAB 2, VANDOEUVRE LES NANCY, FRANCE

[3] AARHUS UNIV HOSP, DK-8000 AARHUS, DENMARK

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1175卷 / 01期

关键词：

TRANSCOBALAMIN; TRANSCOBALAMIN-II RECEPTOR; COBALAMIN; ENDOCYTOSIS; (GERM CELL);

D O I：

10.1016/0167-4889(92)90019-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Specific binding sites for rabbit transcobalamin 11 have been found on isolated adult rabbit germ cells. Scatchard analysis revealed a single class of binding sites for [Co-57]cyanocobalamin-transcobalamin II with an association constant (K(a)) of 1.3 . 10(10) M-1 and 700 sites per cell. Binding was reversible, saturable and calcium dependent. Electron microscope radioautography following incubation with iodinated transcobalamin II at 4-degrees-C led to a detectable labeling mainly restricted to the plasma membrane.

引用

页码：128 / 131

页数：4

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