TRANSCOBALAMIN-II - COBALAMIN BINDING-SITES ARE PRESENT ON RABBIT GERM-CELLS

被引：6

作者：

BOUKHZER, E

ENNYA, A

FELDEN, F

GERARD, A

NEXO, E

NICOLAS, JP

GERARD, H

GUEANT, JL

机构：

[1] FAC MED VANDOEUVRE NANCY, INSERM, U308, BIOCHIM NUTR LAB, F-54500 VANDOEUVRE LES NANCY, FRANCE

[2] FAC MED VANDOEUVRE NANCY, HISTOL EMBRYOL LAB 2, VANDOEUVRE LES NANCY, FRANCE

[3] AARHUS UNIV HOSP, DK-8000 AARHUS, DENMARK

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1175卷 / 01期

关键词：

TRANSCOBALAMIN; TRANSCOBALAMIN-II RECEPTOR; COBALAMIN; ENDOCYTOSIS; (GERM CELL);

D O I：

10.1016/0167-4889(92)90019-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Specific binding sites for rabbit transcobalamin 11 have been found on isolated adult rabbit germ cells. Scatchard analysis revealed a single class of binding sites for [Co-57]cyanocobalamin-transcobalamin II with an association constant (K(a)) of 1.3 . 10(10) M-1 and 700 sites per cell. Binding was reversible, saturable and calcium dependent. Electron microscope radioautography following incubation with iodinated transcobalamin II at 4-degrees-C led to a detectable labeling mainly restricted to the plasma membrane.

引用

页码：128 / 131

页数：4

共 25 条

[11] SERTOLI CELL ORIGIN OF TESTICULAR ANDROGEN-BINDING PROTEIN (ABP)
HAGENAS, L
RITZEN, EM
PLOEN, L
HANSSON, V
FRENCH, FS
NAYFEH, SN
[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1975, 2 (05) : 339 - 350
[12] SYNTHESIS OF TRANSCOBALAMIN-II BY CULTURED HUMAN HEPATOCYTES
HALL, CA
GREENCOLLIGAN, PD
BEGLEY, JA
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 838 (03) : 387 - 389
[13] TRANSFERRIN BINDS SPECIFICALLY TO PACHYTENE SPERMATOCYTES
HOLMES, SD
BUCCI, LR
LIPSHULTZ, LI
SMITH, RG
[J]. ENDOCRINOLOGY, 1983, 113 (05) : 1916 - 1918
[14] MASSON C, 1990, REGARD BIOCH, V4, P27
[15] PURIFICATION AND CHARACTERIZATION OF RABBIT TRANSCOBALAMIN-2
NEXO, E
OLESEN, H
BUCHER, D
THOMSEN, J
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 494 (02) : 395 - 402
[16] CHARACTERIZATION OF THE PARTICULATE AND SOLUBLE ACCEPTOR FOR TRANSCOBALAMIN II FROM HUMAN-PLACENTA AND RABBIT LIVER
NEXO, E
HOLLENBERG, MD
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 628 (02) : 190 - 200
[17] RACHMILEWITZ B, 1977, BIOMED EXPRESS, V27, P213
[18] VITAMIN B12 DEPENDENT METHYLMALONICACIDURIA - DEFECTIVE B12 METABOLISM IN CULTURED FIBROBLASTS
ROSENBERG, LE
LILLJEQVIST, AC
HSIA, YE
ROSENBLOOM, FM
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1969, 37 (04) : 607 - +
[19] BIOSYNTHESIS OF TRANSCOBALAMIN-2 BY ADULT RAT-LIVER PARENCHYMAL-CELLS IN CULTURE
SAVAGE, CR
GREEN, PD
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1976, 173 (02) : 691 - 702
[20] SYNTHESIS AND SECRETION OF A COBALAMIN-BINDING PROTEIN BY HT-29 CELL-LINE
SCHOHN, H
GUEANT, JL
GIRR, M
NEXO, E
BARICAULT, L
ZWEIBAUM, A
NICOLAS, JP
[J]. BIOCHEMICAL JOURNAL, 1991, 280 : 427 - 430

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