RADIOCARBON DATING OF PEPTIDES ISOLATED FROM CONTAMINATED FOSSIL BONE-COLLAGEN BY COLLAGENASE DIGESTION AND REVERSED-PHASE CHROMATOGRAPHY

被引：29

作者：

VANKLINKEN, GJ

BOWLES, AD

HEDGES, REM

机构：

[1] Radiocarbon Accelerator Unit, Research Laboratory for Archaeology and the History of Art, Oxford, OX1 3QJ

来源：

GEOCHIMICA ET COSMOCHIMICA ACTA | 1994年 / 58卷 / 11期

关键词：

D O I：

10.1016/0016-7037(94)90030-2

中图分类号：

P3 [地球物理学]; P59 [地球化学];

学科分类号：

0708 ; 070902 ;

摘要：

The peptide mixture, resulting from collagenase (enzymatic) cleavage of fossil bone collagen, is separated into a fraction containing the very bone-specific tripeptides glycine-proline-hydroxyproline (GlyProHyp) and glycine-proline-alanine (GlyProAla), and more hydrophobic fractions, using reversed-phase chromatography. The suitability of these fractions for C-14 bone dating and stable isotope analysis is discussed, firstly by assessing the theoretical benefits of their use as compared to other bone-specific extracts, and secondly by examining C-14 ages of the fractions in a selection of bones that had previously been giving dubious results using the Oxford routine bone pretreatment. In most cases, the age obtained on the tripeptides GlyProHyp and GlyProAla was in better agreement with the expected age than the original C-14 measurement. In some cases the ages of the fractions were similar, indicating more of an archaeological than a radiocarbon dating problem. We conclude that the use of GlyProHyp/GlyProAla is the best solution to date for C-14 dating of contaminated bone samples.

引用

页码：2543 / 2551

页数：9

共 41 条

[21] PREFERENTIAL PRESERVATION OF NONCOLLAGENOUS PROTEIN DURING BONE DIAGENESIS - IMPLICATIONS FOR CHRONOMETRIC AND STABLE ISOTOPIC MEASUREMENTS [J].

MASTERS, PM .

GEOCHIMICA ET COSMOCHIMICA ACTA, 1987, 51 (12) :3209-3214

[22]

Meister A., 1965, BIOCH AMINO ACIDS, V1

[23] A NEW METHOD FOR CARBON ISOTOPIC ANALYSIS OF PROTEIN [J].

NELSON, DE .

SCIENCE, 1991, 251 (4993) :552-554

[24] THE ISOLATION OF ALLOHYDROXY-L-PROLINE FROM SANDAL (SANTALUM-ALBUM L) [J].

RADHAKRISHNAN, AN ;

GIRI, KV .

BIOCHEMICAL JOURNAL, 1954, 58 (01) :57-61

[25]

ROBINS SP, 1982, COLLAGEN HLTH DISEAS, P160

[26]

SEIFTER S, 1970, METHOD ENZYMOL, V19, P613

[27] ISOLATION, CHARACTERIZATION AND SYNTHESIS OF 3-HYDROXYPROLINES [J].

SHEEHAN, JC ;

WHITNEY, JG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1962, 84 (20) :3980-&

[28] ACCELERATOR RADIOCARBON DATING AT THE MOLECULAR-LEVEL [J].

STAFFORD, TW ;

HARE, PE ;

CURRIE, L ;

JULL, AJT ;

DONAHUE, DJ .

JOURNAL OF ARCHAEOLOGICAL SCIENCE, 1991, 18 (01) :35-72

[29] ISOLATION OF PROLINE AND HYDROXYPROLINE FROM FOSSIL BONE [J].

STAFFORD, TW ;

DUHAMEL, RC ;

HAYNES, CV ;

BRENDEL, K .

LIFE SCIENCES, 1982, 31 (09) :931-938

[30]

STEINBRINK DR, 1981, FED PROC, V40, P1718

← 1 2 3 4 5 →