ELECTROTRANSFECTION OF PROPIONIBACTERIUM-FREUDENREICHII TL-110

被引：11

作者：

GAUTIER, M

ROUAULT, A

LEMEE, R

机构：

[1] Laboratoire de Recherches de Technologie Laitiére, INRA, Rennes

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1995年 / 20卷 / 02期

关键词：

D O I：

10.1111/j.1472-765X.1995.tb01302.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The first highly efficient protocol is described for the electrotransfection of Propionibacterium freudenreichii with DNA phage. The transfection efficiency is 7 x 10(5) transfectants per mu g of DNA under optimal conditions. Optimized parameters included the field strength (12.5 kV, 200 Ohms, 25 mu F), phage DNA concentration (1 mu g ml(-1)) and cell density (1.5 x 10(10) cells ml(-1)). Growth in the presence of glycine and harvesting of cells during the early exponential growth phase increased the transfection efficiency. This electrotransfection protocol is of importance for the genetic improvement of dairy propionibacteria.

引用

页码：125 / 129

页数：5

共 18 条

[1] SIMPLE AND RAPID METHOD FOR ISOLATING LARGE PLASMID DNA FROM LACTIC STREPTOCOCCI [J].