COMPLEMENT ACTIVATION BY WHOLE ENDOTOXIN IS BLOCKED BY A MONOCLONAL-ANTIBODY TO FACTOR-B

Sepsis is both a common and, despite present-day therapy, a serious disease. The pathophysiology of the septic response is a complex, multifactorial phenomenon which in part involves the activation of complement by bacterial endotoxin. A monoclonal antibody to human complement factor B, code-named 1H5, which was capable of specifically inhibiting the alternative pathway of complement activation at concentrations as low as 1 mu g/ml, is described. This agent had no effect on the classical pathway of complement activation. It was capable of preventing the activation of complement by even high concentrations (0.1 mg/ml) of whole endotoxin; however, it was ineffective in preventing activation of complement by endotoxin derived front a rough mutant. This agent could potentially be used in the treatment of sepsis.