A METHODICAL STUDY OF SHAPE CHANGES IN HUMAN ORAL CELLS PERTURBED BY A SIMULATED ORTHODONTIC STRAIN IN-VITRO

Cells are known to alter their shape as a response to physical and chemical changes. Mechanical loads applied to teeth produce cellular perturbations resulting in orthodontic movement. An in vitro model was developed to simulate the in vivo strain of orthodontic movement. Calibrated forces were applied to human periodontal ligament cells and buccal mucosal fibroblasts (controls). A biaxial strain-producing device was used to stretch vital cells grown on flexible polyletrafluorethylene membranes. In addition, a new cell adhesive, Cell Tak(TM), was employed to examine the effect of an adhesive substrate on the cellular response to two known loads. The shape changes of unstrained (control) and strained cells were evaluated by time-lapse telemicroscopy, and plots of time-dependent alterations in area and shape were recorded. The fusiform cells became more rounded over a given time of up to 1400 s. The responses appeared to be independent of cell type, the strain employed, and the presence of cell adhesive. Scanning electron microscopy demonstrated, irrespective of cell type, that the surface of stressed cells produced a striking number of microvilli as compared with the relatively smooth-surfaced controls.