CONSTRUCTION OF GENETICALLY MARKED RUMINOCOCCUS-ALBUS STRAINS AND CONJUGAL TRANSFER OF PLASMID PAM-BETA-1 INTO THEM

被引：9

作者：

AMINOV, RI ^{[1
]}

KANEICHI, K ^{[1
]}

MIYAGI, T ^{[1
]}

SAKKA, K ^{[1
]}

OHMIYA, K ^{[1
]}

机构：

[1] MIE UNIV,FAC BIORESOURCES,TSU,MIE 514,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1994年 / 78卷 / 01期

基金：

日本学术振兴会;

关键词：

D O I：

10.1016/0922-338X(94)90169-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Anaerobic filter matings of Ruminococcus albus F40/2 and A2, both rifampicin- and streptomycin-resistant mutants, were done with Enterococcus faecalis DS16C3, which contains chromosomally inserted Tn 916 and plasmid pAMbeta1, and with Bacillus thuringiensis BT351, which contains pAMbeta1. Erythromycin-resistant transconjugants were obtained in mating of R. albus A2 and B. thuringiensis BT351 with an average frequency of 7 x 10(-8) (per recipient). The identity of plasmid pAMbeta1 in R. albus A3 transconjugants was confirmed by restriction and Southern hybridization analyses.

引用

收藏

页码：1 / 5

页数：5

相关论文

共 30 条

[1] BIOTECHNOLOGY AND THE RUMEN - A MINI REVIEW [J].

ARMSTRONG, DG ;

GILBERT, HJ .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1985, 36 (11) :1039-1046

[2] ISOLATION AND CHARACTERIZATION OF PLASMID DNA FROM RUMINOCOCCUS [J].

ASMUNDSON, RV ;

KELLY, WJ .

CURRENT MICROBIOLOGY, 1987, 16 (02) :97-100

[3] LINKAGE MAP OF ESCHERICHIA-COLI K-12, EDITION-8 [J].

BACHMANN, BJ .

MICROBIOLOGICAL REVIEWS, 1990, 54 (02) :130-197

[4] UNIDIRECTIONAL THETA REPLICATION OF THE STRUCTURALLY STABLE ENTEROCOCCUS-FAECALIS PLASMID PAM-BETA-1 [J].

BRUAND, C ;

EHRLICH, SD ;

JANNIERE, L .

EMBO JOURNAL, 1991, 10 (08) :2171-2177

[5] MEDIUM WITHOUT RUMEN FLUID FOR NONSELECTIVE ENUMERATION AND ISOLATION OF RUMEN BACTERIA [J].

CALDWELL, DR ;

BRYANT, MP .

APPLIED MICROBIOLOGY, 1966, 14 (05) :794-&

[6] ANALYSIS OF ANTIBIOTIC SUSCEPTIBILITY AND EXTRACHROMOSOMAL DNA CONTENT OF RUMINOCOCCUS-ALBUS AND RUMINOCOCCUS-FLAVEFACIENS [J].

CHAMPION, KM ;

HELASZEK, CT ;

WHITE, BA .

CANADIAN JOURNAL OF MICROBIOLOGY, 1988, 34 (10) :1109-1115

[7]

COCCONCELLI PS, 1992, FEMS MICROBIOL LETT, V94, P203, DOI 10.1111/j.1574-6968.1992.tb05319.x

[8] PURIFICATION AND PROPERTIES OF THE ENDO-1,4-BETA-GLUCANASE FROM RUMINOCOCCUS-ALBUS AND ITS GENE-PRODUCT IN ESCHERICHIA-COLI [J].

DEGUCHI, H ;

WATANABE, Y ;

SASAKI, T ;

MATSUDA, T ;

SHIMIZU, S ;

OHMIYA, K .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1991, 71 (04) :221-225

[9] POTENTIAL FOR MANIPULATION OF THE RUMEN FERMENTATION THROUGH THE USE OF RECOMBINANT-DNA TECHNIQUES [J].

FORSBERG, CW ;

CROSBY, B ;

THOMAS, DY .

JOURNAL OF ANIMAL SCIENCE, 1986, 63 (01) :310-325

[10] BIOTECHNOLOGY AND MODIFICATIONS OF THE RUMEN MICROBIAL ECOSYSTEM [J].

HESPELL, RB .

PROCEEDINGS OF THE NUTRITION SOCIETY, 1987, 46 (03) :407-413