FORMATION OF TREHALOSE AND ITS 2-DEOXY ANALOGS THROUGH CONDENSATION BY A TREHALASE FROM LOBOSPHAERA SP

被引：19

作者：

NAKANO, H ^{[1
]}

MORIWAKI, M ^{[1
]}

WASHINO, T ^{[1
]}

KITAHATA, S ^{[1
]}

机构：

[1] SAN EI GEN FFI INC, TOYONAKA, OSAKA 561, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 08期

关键词：

D O I：

10.1271/bbb.58.1435

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A purified trehalase from Lobosphaera sp. catalyzed the condensation not only of D-glucose but also of 2-deoxy-D-glucose, yielding alpha,alpha'-trehalose and 2,2'-dideoxy-alpha,alpha'-trehalose, respectively. Simultaneous incubation of D-glucose and 2-deoxy-D-glucose resulted in the formation of 2-deoxy-alpha,alpha'-trehalose in addition to the above disaccharides. The reaction under various conditions showed that the enzyme synthesized dideoxy-trehalose (yield, 16%) more than trehalose (5%). Monodeoxy-trehalose was produced most effectively in the reaction of D-glucose and 2-deoxy-D-glucose in a weight ratio of 2:5, and at this ratio, 6% of the total weight of two substrates was converted to the product.

引用

页码：1435 / 1438

页数：4

共 16 条

[1] ENZYMIC-SYNTHESIS OF DISACCHARIDES BY USE OF THE REVERSED HYDROLYSIS ACTIVITY OF BETA-D-GALACTOSIDASES [J].