FORMATION OF TREHALOSE AND ITS 2-DEOXY ANALOGS THROUGH CONDENSATION BY A TREHALASE FROM LOBOSPHAERA SP

被引：19

作者：

NAKANO, H ^{[1
]}

MORIWAKI, M ^{[1
]}

WASHINO, T ^{[1
]}

KITAHATA, S ^{[1
]}

机构：

[1] SAN EI GEN FFI INC, TOYONAKA, OSAKA 561, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 08期

关键词：

D O I：

10.1271/bbb.58.1435

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A purified trehalase from Lobosphaera sp. catalyzed the condensation not only of D-glucose but also of 2-deoxy-D-glucose, yielding alpha,alpha'-trehalose and 2,2'-dideoxy-alpha,alpha'-trehalose, respectively. Simultaneous incubation of D-glucose and 2-deoxy-D-glucose resulted in the formation of 2-deoxy-alpha,alpha'-trehalose in addition to the above disaccharides. The reaction under various conditions showed that the enzyme synthesized dideoxy-trehalose (yield, 16%) more than trehalose (5%). Monodeoxy-trehalose was produced most effectively in the reaction of D-glucose and 2-deoxy-D-glucose in a weight ratio of 2:5, and at this ratio, 6% of the total weight of two substrates was converted to the product.

引用

页码：1435 / 1438

页数：4

共 16 条

[11] STUDIES OF THE REVERSED ALPHA-MANNOSIDASE REACTION IN HIGH-CONCENTRATIONS OF MANNOSE [J].

JOHANSSON, E ;

HEDBYS, L ;

MOSBACH, K ;

LARSSON, PO ;

GUNNARSSON, A ;

SVENSSON, S .

ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (06) :347-352

[12] EFFECT OF ORGANIC-SOLVENTS ON STABILITY OF 2 GLYCOSIDASES AND ON GLUCOAMYLASE-CATALYZED OLIGOSACCHARIDE SYNTHESIS [J].

LAROUTE, V ;

WILLEMOT, RM .

ENZYME AND MICROBIAL TECHNOLOGY, 1992, 14 (07) :528-534

[13] PURIFICATION AND SOME PROPERTIES OF A TREHALASE FROM A GREEN-ALGA, LOBOSPHAERA SP [J].

NAKANO, H ;

MORIWAKI, M ;

WASHINO, T ;

KINO, TS ;

YOSHIZUMI, H ;

KITAHATA, S .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (08) :1430-1434

[14] GLUCO-OLIGOSACCHARIDE SYNTHESIS BY FREE AND IMMOBILIZED BETA-GLUCOSIDASE [J].

RAVET, C ;

THOMAS, D ;

LEGOY, MD .

BIOTECHNOLOGY AND BIOENGINEERING, 1993, 42 (03) :303-308

[15] TREHALASE IN THE THORACIC MUSCLES OF THE WOODROACH, LEUCOPHAEA MADERAE [J].

ZEBE, EC ;

MCSHAN, WH .

JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY, 1959, 53 (01) :21-29

[16] METABOLISM OF 2-DEOXY-D-GLUCOSE IN BAKERS-YEAST .5. FORMATION OF 2-DEOXY-ALPHA, ALPHA'-TREHALOSE [J].

ZEMEK, J ;

BAUER, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 264 (03) :393-+

← 1 2 →