Metabolites in the urine after administration of deprenyl and methamphetamine were compared. Determination of deprenyl, N-desmethyldeprenyl, methamphetamine, p-hydroxymethamphetamine, amphetamine and p-hydroxy amphetamine in the urine was determined by use of solid extraction, trifluoroacetylation and bis (trimethylsilyl) acetamide treatment, and gas chromatography-mass spectrometry. This method was applied to the determination of metabolites in the urine of monkey administered subcutaneously at 0.2 mg/kg of deprenyl hydrochloride or methamphetamine hydrochloride. Deprenyl and desmethyldeprenyl were detected in the urine only within 12 h after deprenyl administration. Even after their disappearance, the prolonged excretion of methamphetamine and amphetamine, metabolites of deprenyl, were found until 48 h after deprenyl administration. As a result, it was suggested that deprenyl or desmethyldeprenyl in the urine seemed to be used as a marker for the discrimination between deprenyl and methamphetamine use. However, the faster disappearance of deprenyl and desmethyldeprenyl should be taken into consideration. © 1990, The Pharmaceutical Society of Japan. All rights reserved.
