EFFECT OF HYPOPHYSECTOMY ON INSULIN-LIKE GROWTH-FACTOR (IGF)-1 BINDING-ACTIVITY OF SERUM IN CHICKENS

被引：10

作者：

MORISHITA, D ^{[1
]}

WAKITA, M ^{[1
]}

HOSHINO, S ^{[1
]}

机构：

[1] MIE UNIV,FAC BIORESOURCES,TSU,MIE 514,JAPAN

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY | 1993年 / 104卷 / 02期

关键词：

D O I：

10.1016/0300-9629(93)90314-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Hypophysectomy elevated IGF-1 binding activity of serum concurrently with decreases in circulating concentrations of IGF-1 and growth hormone (GH). 2. When sera pooled from each experimental group were chromatographed on a Sephadex G-200 column, IGF-1 binding activity was mainly found in 150 and 30 kilodalton (kDa) proteins. 3. Most of the serum IGF-1 were distributed among 150 kDa protein fractions. 4. Hypophysectomy elevated IGF-1 binding activity of these proteins, especially that of 30 kDa protein. 5. The binding of [I-125]IGF-1 to the 30 kDa protein was inhibited by unlabelled IGF-1 but not by insulin, and saturated with a small amount of the ligand compared to the 150 kDa protein. 6. The IGF-1 binding activity of 30 kDa protein was dependent on GH status of chickens.

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页码：261 / 265

页数：5

相关论文

共 15 条

[1] INSULIN-LIKE GROWTH FACTOR-I BINDING-PROTEINS IN SERUM FROM THE DOMESTIC-FOWL [J].

ARMSTRONG, DG ;

MCKAY, CO ;

MORRELL, DJ ;

GODDARD, C .

JOURNAL OF ENDOCRINOLOGY, 1989, 120 (03) :373-378

[2] GROWTH HORMONE-DEPENDENT INSULIN-LIKE GROWTH-FACTOR (IGF) BINDING-PROTEIN FROM HUMAN-PLASMA DIFFERS FROM OTHER HUMAN IGF BINDING-PROTEINS [J].

BAXTER, RC ;

MARTIN, JL ;

TYLER, MI ;

HOWDEN, MEH .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 139 (03) :1256-1261

[3] RADIOIMMUNOASSAY OF GROWTH-HORMONE DEPENDENT INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN IN HUMAN-PLASMA [J].

BAXTER, RC ;

MARTIN, JL .

JOURNAL OF CLINICAL INVESTIGATION, 1986, 78 (06) :1504-1512

[4] INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEINS [J].

CLEMMONS, DR .

TRENDS IN ENDOCRINOLOGY AND METABOLISM, 1990, 1 (08) :412-417

[5] PLASMA-CLEARANCE OF CHICKEN AND HUMAN INSULIN-LIKE GROWTH FACTOR-I AND THEIR ASSOCIATION WITH CIRCULATING BINDING-PROTEINS IN CHICKENS [J].

FRANCIS, GL ;

MCMURTRY, JP ;

JOHNSON, RJ ;

BALLARD, FJ .

JOURNAL OF ENDOCRINOLOGY, 1990, 124 (03) :361-370

[6] PLASMA-CONCENTRATIONS OF SOMATOMEDIN-C IN HYPOPHYSECTOMIZED, DWARF AND INTACT GROWING DOMESTIC-FOWL AS DETERMINED BY HETEROLOGOUS RADIOIMMUNOASSAY [J].

HUYBRECHTS, LM ;

KING, DB ;

LAUTERIO, TJ ;

MARSH, J ;

SCANES, CG .

JOURNAL OF ENDOCRINOLOGY, 1985, 104 (02) :233-239

[7] EFFECT OF HYPOPHYSECTOMY OF YOUNG COCKERELS WITH PARTICULAR REFERENCE TO BODY GROWTH LIVER WEIGHT AND LIVER GLYCOGEN LEVEL [J].

KING, DB .

GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1969, 12 (02) :242-&

[8] ACUTE EFFECTS OF HYPOPHYSECTOMY AND ADMINISTRATION OF PANCREATIC AND THYROID-HORMONES ON CIRCULATING CONCENTRATIONS OF SOMATOMEDIN-C IN YOUNG CHICKENS - RELATIONSHIP BETWEEN GROWTH-HORMONE AND SOMATOMEDIN-C [J].

LAZARUS, DD ;

SCANES, CG .

DOMESTIC ANIMAL ENDOCRINOLOGY, 1988, 5 (04) :283-289

[9] INSULIN-LIKE GROWTH-FACTOR (IGF)-I AND IGF-BINDING ACTIVITY IN NORMAL AND FAST-GROWING CHICKENS [J].

LEE, PDK ;

PEACOCK, A ;

ROESSLER, MK ;

HESTER, J ;

REEVES, JT .

LIFE SCIENCES, 1989, 45 (25) :2465-2470

[10]

MORISHITA D, 1991, P JAPAN SOC COMP END, V6, P59