BINDING OF SOLUBLE TYPE-1 COLLAGEN MOLECULES TO THE FIBROBLAST PLASMA-MEMBRANE

Soluble 125I-labeled type I collagen binds to cultured fibroblasts but not to cultured epithelia. The binding of the ligand to fibroblasts is reversible, saturable and highly specific for sequences contained within the helical portions of the α1 and α2 chains. The amount of ligand bound is dependent upon cell number and ligand concentration. Binding is decreased but measurable at 4°C. The steady state binding is greater at 26° than at 37°C due to a more rapid dissociation of the ligand-acceptor complex at 37°C. The half-life of the complex is 46 min at 37°C and approximately 2.5 hr at 26°C. Scatchard plots of binding data indicate a single class of high affinity binding sites (KD = 1.2 × 10-11 M) with each fibroblast binding approximately 500,000 molecules at saturation. Pretreatment of fibroblasts with bacterial collagenase, chondroitinase ABC or testicular hyaluronidase does not affect the binding reaction, whereas pretreatment of the cells with phospholipase C increases the amount of ligand bound. Ligand binding is decreased but not abolished after fibroblasts are treated with trypsin concentrations which remove surface fibronectin. Fibroblast monolayers treated with antiserum against fibronectin bind the radiolabeled ligand normally. In contrast to collagen, addition of excess fibronectin does not accelerate the dissociation of bound ligand from fibroblasts. Possible functions for surface-bound collagen are discussed. © 1979.