共 25 条
SPONTANEOUS AND AGONIST-INDUCED OPENINGS OF AN ACETYLCHOLINE-RECEPTOR CHANNEL COMPOSED OF BOVINE MUSCLE ALPHA-SUBUNIT, BETA-SUBUNIT AND DELTA-SUBUNIT
被引:48
作者:
JACKSON, MB
IMOTO, K
MISHINA, M
KONNO, T
NUMA, S
SAKMANN, B
机构:
[1] MAX PLANCK INST MED RES,ZELLPHYSIOL ABT,W-6900 HEIDELBERG 1,GERMANY
[2] KYOTO UNIV,FAC MED,DEPT MED CHEM,KYOTO 606,JAPAN
[3] KYOTO UNIV,FAC MED,DEPT MOLEC GENET,KYOTO 606,JAPAN
来源:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
|
1990年
/
417卷
/
02期
关键词:
Acetylcholine receptor;
channel;
single;
Xenopus oocyte;
D O I:
10.1007/BF00370689
中图分类号:
Q4 [生理学];
学科分类号:
071003 ;
摘要:
During the development of mammalian muscle the γ-subunit of the nicotinic acetylcholine receptor (AChR) is replaced by the e{open}-subunit to produce well-defined alterations in the conductance and gating of the channel. To gain a better unterstanding of the functional role of the γ and e{open}-subunits, we have studied the properties of an AChR channel lacking these subunits. The AChR expressed in Xenopus oocytes injected with the bovine α-, β and δ-subunit-specific mRNAs (referred to as αβδ-AChR) is unusual in that its channel opens spontaneously at a high frequency in the absence of agonist. From a comparison of the αβδ-AChR with complete receptors containing either the γ or e{open}-subunit, we conclude that the γ and e{open}-subunits influence most channel properties, including agonist binding, and are especially important for stabilizing the closed state of the unliganded receptor channel. The αβδ-AChR can form when a complete set of four subunit-specific mRNAs is injected. The ease with which it is assembled raises the possibility that the αβδ- AChR contributes to some of the variations in receptor properties that occur during development. © 1990 Springer-Verlag.
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页码:129 / 135
页数:7
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