ESCHERICHIA-COLI GAMMA-GLUTAMYL-TRANSPEPTIDASE MUTANTS DEFICIENT IN PROCESSING TO SUBUNITS

被引：17

作者：

HASHIMOTO, W

SUZUKI, H

NOHARA, S

KUMAGAI, H

机构：

[1] Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)91540-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Arginyl residues 513 and 571 of Escherichia coli K-12 γ-glutamyltranspeptidase (EC 2. 3. 2. 2.) were substituted with alanyl and glycyl residues, respectively, by oligonucleotide-directed in vitro mutagenesis. Both mutants were devoid of the enzymatic activity. On Western blot analysis, we found that both mutants accumulated a γ-glutamyltranspeptidase precursor which was not processed into large and small subunits in the periplasmic space of Escherichia coli. © 1992.

引用

页码：173 / 178

页数：6

共 25 条

[1] POSITIVE SELECTION FOR LOSS OF TETRACYCLINE RESISTANCE [J].