FORMATION IN-VITRO OF THE 3,4,6-TRIHYDROXYPHENYLALANINE QUINONE COFACTOR

被引：15

作者：

HANLON, SP ^{[1
]}

CARPENTER, K ^{[1
]}

HASSAN, A ^{[1
]}

COOPER, RA ^{[1
]}

机构：

[1] UNIV LEICESTER,DEPT BIOCHEM,LEICESTER LE1 7RH,LEICS,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1995年 / 306卷

关键词：

D O I：

10.1042/bj3060627

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An Escherichia coli K-12 2-phenylethylamine oxidase gene with a mutated leader sequence region produced a largely inactive form of the enzyme in the cytoplasm. This form of the enzyme was activated 30-50-fold on incubation at 30 degrees C in the absence of any added cofactors. After activation the enzyme contained a quinone which was not detected in the non-activated form. This is the first report of the formation in vitro of any quinoenzyme cofactor.

引用

页码：627 / 630

页数：4

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