A METHOD USING DII TO STUDY THE CONNECTIVITY OF CORTICAL TRANSPLANTS

被引：16

作者：

HEREDIA, M

SANTACANA, M

VALVERDE, F

机构：

[1] Instituto Cajal, Madrid

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1991年 / 36卷 / 01期

关键词：

CARBOCYANINE DII; CORTICAL TRANSPLANTS; CONNECTIVITY;

D O I：

10.1016/0165-0270(91)90133-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Carbocyanine DiI is described as a suitable fluorescent tracer to investigate the connectivity of cortical transplants. DiI was applied in 2 ways: in tissue previously fixed with aldehydes, and in vivo for labelling the donor tissue prior to transplantation. When applied in fixed tissue DiI travelled anterogradely, allowing the study of efferent connections from the transplant to the host. When DiI was applied in vivo, it travelled retrogradely showing the afferents to the transplant from the host.

引用

页码：17 / 25

页数：9

共 21 条

[1] AXONAL PATHFINDING DURING THE REGENERATION OF THE GOLDFISH OPTIC PATHWAY [J].