MYCOSIS-FUNGOIDES EXHIBITS A TH1-TYPE CELL-MEDIATED CYTOKINE PROFILE WHEREAS SEZARY-SYNDROME EXPRESSES A TH2-TYPE PROFILE

被引:152
作者
SAED, G
FIVENSON, DP
NAIDU, Y
NICKOLOFF, BJ
机构
[1] HENRY FORD HOSP,DEPT DERMATOL,DETROIT,MI 48202
[2] UNIV MICHIGAN,SCH MED,DEPT PATHOL,ANN ARBOR,MI
关键词
CUTANEOUS T-CELL LYMPHOMA; CYTOKINES; PSORIASIS; INTERLEUKINS; GAMMA INTERFERON; PCR;
D O I
10.1111/1523-1747.ep12388985
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
We determined T-cell cytokine profiles in the epidermis, dermis, and blood of cutaneous T-cell lymphoma to differentiate whether unique cytokine profiles were associated with mycosis fungoides (MF) versus Sezary syndrome. Punch biopsy specimens from plaque stage MF (n = 7) were compared to Sezary skin (n = 3) after undergoing rapid heat-saline separation of epidermis from dermis. Normal adult skin (n = 11), neonatal foreskin (n = 4), untreated psoriatic plaques (n = 6), and normal donor peripheral blood leukocytes (n = 3) were studied as controls. Total RNA was extracted from all skin specimens, as well as peripheral blood leukocytes from MF (n = 3) and Sezary patients (n = 7), and was converted to cDNA by reverse transcriptase. Polymerase chain reaction amplification of cDNAs using interleukin 2 (IL-2), IL-4, IL-5, IL-10, and interferon gamma- specific primers was used to differentiate Th1-type responses (IL-2+ and interferon gamma+) from Th2-type responses (IL-4+, IL-5+, and IL-10+). beta-actin specific primers were included as a positive control for mRNA integrity. All MF specimens contained mRNAs for IL-2 and interferon gamma limited to epidermis but no IL-4, IL-5, or IL-10. In contrast, Sezary skin and blood showed a cytokine mRNA pattern dominated by IL-4, IL-5, and IL-10.;MF blood showed a pattern similar to normal peripheral blood T cells with mixed detection of all T-helper cell cytokine mRNAs. All psoriasis samples contained mRNAs for IL-2 and interferon gamma in both epidermis and dermis with no IL-4 or IL-10 in either compartment. These findings demonstrate that the cutaneous lesions of MF are characterized by an epidermal Th1-type cytokine profile, whereas both the blood and skin of patients with Sezary syndrome is characterized by a Th2-type profile. This work suggests that differences in cytokine production may be related to the pathophysiology and clinical presentation in cutaneous T-cell lymphoma.
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页码:29 / 33
页数:5
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