EVIDENCE FOR AN INVOLVEMENT OF THYMIDINE KINASE IN THE EXCISION REPAIR OF ULTRAVIOLET-IRRADIATED HERPES-SIMPLEX VIRUS IN HUMAN-CELLS

被引：4

作者：

INTINE, RVA

RAINBOW, AJ

机构：

[1] MCMASTER UNIV,DEPT BIOL,HAMILTON L8S 4K1,ONTARIO,CANADA

[2] MCMASTER UNIV,DEPT RADIOL,HAMILTON L8S 4K1,ONTARIO,CANADA

来源：

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 1990年 / 15卷 / 01期

关键词：

DNA repair; reactivation of virus; xeroderma pigmentosum;

D O I：

10.1002/em.2850150104

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A wild‐type strain of herpes simplex virus type 1 (HSV‐1:KOS) encoding a functional thymidine kinase (tk+) and a tk‐ mutant strain (HSV‐1:PTK3B) were used to study the role of the viral tk in the repair of UV‐irradiated HSV‐1 in human cells. UV survival of HSV‐1:PTK3B was substantially reduced compared with that of HSV‐1:KOS when infecting normal human cells. In contrast, the UV survival of HSV‐1:PTK3B was similar to that of HSV‐1:KOS when infecting excision repair‐deficient cells from a xeroderma pigmentosum patient from complementation group A. These results suggest that the repair of UV‐irradiated HSV‐1 in human cells depends, in part at least, on expression of the viral tk and that the repair process influenced by tk activity is excision repair or a process dependent on excision repair. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：19 / 23

页数：5

共 35 条

[31] CONSEQUENCES OF THE DEPLETION OF CELLULAR DEOXYNUCLEOSIDE TRIPHOSPHATE POOLS ON THE EXCISION-REPAIR PROCESS IN CULTURED HUMAN-FIBROBLASTS [J].