CHARACTERIZATION OF AN ANTHER-SPECIFIC AND TAPETUM-SPECIFIC GENE ENCODING A GLYCINE-RICH PROTEIN FROM TOMATO

被引:14
作者
CHEN, RD [1 ]
AGUIRRE, PJ [1 ]
SMITH, AG [1 ]
机构
[1] UNIV MINNESOTA,INST PLANT MOLEC GENET,ST PAUL,MN 55108
关键词
LYCOPERSICON ESCULENTUM; CDNA SEQUENCE; FLOWER; LOCALIZATION; STAMEN;
D O I
10.1016/S0176-1617(11)81153-3
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A cDNA clone, TomA92B9, encoding a putative 15-kDa glycine-rich protein from tomato anthers, was isolated and characterized. The gene is present in one copy per haploid genome. The mRNA accumulated predominantly in the tapetal layer of stamens from early meiosis through tetrad release. The protein deduced from this clone has an N-terminal hydrophobic domain with the characteristics of a secretory signal. The mature protein contains 32% of Gly residues. On the basis of the analysis of gene's expression pattern and its primary structure, it is concluded that this gene product is a novel glycine-rich protein.
引用
收藏
页码:651 / 658
页数:8
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