BOMBESIN ACTS TO SUPPRESS FEEDING-BEHAVIOR AND ALTER SERUM GROWTH-HORMONE IN GOLDFISH

The acute effects of a single injection of bombesin (BBS) on feeding behavior and serum growth hormone (GH) levels in goldfish were examined. When injected intraperitoneally (IP), BBS (0.5- 100 ng/g) caused a dose-dependent decrease in food intake within 30 and 45 min of administration; maximal suppression was achieved at 50 ng/g BBS and was accompanied by an elevation in serum GH levels. Associated with IP injection of BBS was a pronounced spitting out behavior in which food pellets were taken into the oral cavity but immediately expelled. When injected into goldfish deprived of food for 72 h, 50 ng/g BBS was still potent in suppressing feeding behavior and increasing serum GH. Additionally, IP injection of BBS (10 or 100 ng/g) into groups of fish caused a significant increase in circulating serum GH levels at 1.5 h postinjection. Finally, when injected into the third brain ventricle (ICV), 60 ng/g BBS also caused a suppression in food intake and a concomitant increase in serum GH. Groups of fish injected ICV with 5 or 50 ng/g BBS also exhibited a graded increase in serum GH levels at 45 min postinjection. Overall, these data are the first to demonstrate in any lower vertebrate that a neuropeptide acts to suppress food intake and cause concomitant alterations in circulating serum GH levels, following either peripheral or central administration.