HETEROGENEITY OF DROSOPHILA NICOTINIC ACETYLCHOLINE-RECEPTORS - SAD, A NOVEL DEVELOPMENTALLY REGULATED ALPHA-SUBUNIT

被引：77

作者：

SAWRUK, E ^{[1
]}

SCHLOSS, P ^{[1
]}

BETZ, H ^{[1
]}

SCHMITT, B ^{[1
]}

机构：

[1] MAX PLANCK INST HIRNFORSCH,W-6000 FRANKFURT 71,GERMANY

来源：

EMBO JOURNAL | 1990年 / 9卷 / 09期

关键词：

developmental regulation; drosophila melanogaster; nicotinic acetylcholine receptor; receptor heterogeneity;

D O I：

10.1002/j.1460-2075.1990.tb07452.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two genes, ard and als, are known to encode subunits of the nicotinic acetylcholine receptor (nAChR) in Drosophila. Here we describe the isolation of cDNA clones encoding a novel member (SAD, or α2) of this receptor protein family. The deduced amino acid sequence displays high homology to the ALS protein and shares structural features with ligand binding nAChR α-subunits. Sad transcripts accumulate during major periods of neuronal differentiation and, in embryos, are localized in the central nervous system. Expression of SAD cRNA in Xenopus oocytes generates cation channels that are gated by nicotine. These data indicate heterogeneity of nAChRs in Drosophila.

引用

页码：2671 / 2677

页数：7