DIFFERENTIAL PROCESSING OF THE NEUROTENSIN NEUROMEDIN-N PRECURSOR IN THE MOUSE

被引:35
作者
SHAW, C
MCKAY, D
JOHNSTON, CF
HALTON, DW
FAIRWEATHER, I
KITABGI, P
BUCHANAN, KD
机构
[1] QUEENS UNIV BELFAST,DEPT MED,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND
[2] QUEENS UNIV BELFAST,DEPT BIOL,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND
[3] UNIV NICE,FAC SCI,CTR BIOCHEM,F-06034 NICE,FRANCE
关键词
Enzymatic digestion; Mouse; Neuromedin N; Neurotensin; Precursor processing; Region-specific radioimmunoassay; Reverse phase HPLC;
D O I
10.1016/0196-9781(90)90075-G
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Posttranslational processing of the neurotensin/neuromedin N (NT/NN) precursor has been investigated in mouse brain and small intestine by means of region-specific radioimmunoassays coupled to chromatographic fractionations. In brain, total NT/NN immunoreactivity measured with a common C-terminal antiserum was 15.72 pmol/g. NT measured with an N-terminal antiserum was 9.74 pmol/g and NN measured with an N-terminal antiserum was 5.98 pmol/g. In small intestine, combined NT/NN immunoreactivity was 108.55 pmol/g, consisting of 66.37 pmol/g NT but only 0.96 pmol/g NN. Gel permeation chromatography and reverse phase HPLC revealed that the large discrepancy in the NT and NN values obtained in small intestinal extracts was due to the presence of a high molecular weight, hydrophobic peptide, which was reactive only with the common C-terminally directed antiserum. Pepsinization of this generated an immunoreactive peptide with similar chromatographic characteristics to NN. In mouse intestine, NN is only partially cleaved from the common NT/NN precursor, resulting in the presence of an N-terminally extended molecular species. This novel molecular species of neuromedin N may be the physiological mediator of certain peripheral biological effects hitherto attributed to neurotensin or neuromedin N. © 1990.
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页码:227 / 235
页数:9
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