A CONTIG OF NON-CHIMERIC YACS CONTAINING THE SPINAL MUSCULAR-ATROPHY GENE IN 5Q13

被引：72

作者：

FRANCIS, MJ

MORRISON, KE

CAMPBELL, L

GREWAL, PK

CHRISTODOULOU, Z

DANIELS, RJ

MONACO, AP

FRISCHAUF, AM

MCPHERSON, J

WASMUTH, J

DAVIES, KE

机构：

[1] JOHN RADCLIFFE HOSP,INST MOLEC MED,MOLEC GENET GRP,OXFORD OX3 9DU,ENGLAND

[2] JOHN RADCLIFFE HOSP,INST MOLEC MED,IMPERIAL CANC RES FUND,OXFORD OX3 9DU,ENGLAND

[3] IMPERIAL CANC RES FUND,LONDON WC2A 3PX,ENGLAND

[4] UNIV CALIF IRVINE,DEPT BIOL CHEM,IRVINE,CA 92717

来源：

HUMAN MOLECULAR GENETICS | 1993年 / 2卷 / 08期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/hmg/2.8.1161

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed a contig of non-chimaeric yeast artificial chromosomes (YACs) across the candidate region for childhood autosomal recessive spinal muscular atrophy (SMA) in 5q13. A novel microsatellite reduces the candidate region to approximately 400kb of DNA distal to D5S435. The candidate region contains blocks of chromosome 5 specific repeats which have copies on 5p as well as elsewhere on 5q. Restriction mapping of the YACs reveals at least one CpG island in the SMA gene region. The YAC maps indicate that the contig contains minimal rearrangements or deletions. The data show the value of screening several YAC libraries simultaneously in order to construct a set of overlapping sequences suitable for candidate gene searches and direct genomic sequencing.

引用

页码：1161 / 1167

页数：7

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