ENANTIOSELECTIVE HYDROLYSIS OF (RS)-2-ISOPROPYL-4'-CHLOROPHENYLACETONITRILE BY PSEUDOMONAS SP B21C9

被引：12

作者：

MASUTOMO, S

INOUE, A

KUMAGAI, K

MURAI, R

MITSUDA, S

机构：

[1] Biotechnology Laboratory, Sumitomo Chemical Co., Takarazuka, Hyogo 665

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 04期

关键词：

D O I：

10.1271/bbb.59.720

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pseudomonas sp, B21C9, a new nitrile-degrading microorganism, was isolated from a soil sample. By the hydrolysis of (RS)-isopropyl-4'-chlorophenylacetonitrile using cells of B21C9, (S)-2-isopropyl-4'-chlorophenylacetic acid having excellent optically purity could be obtained, It appeared that the microbial hydrolysis proceeded via stepwise reactions by a nitrile hydratase having poor (S)-selectivity and an amidase having strict (S)-selectivity.

引用

收藏

页码：720 / 722

页数：3

相关论文

共 22 条

[1] MICROBIAL-DEGRADATION OF NITRILE COMPOUNDS .7. A NEW ENZYMATIC METHOD OF ACRYLAMIDE PRODUCTION [J].

ASANO, Y ;

YASUDA, T ;

TANI, Y ;

YAMADA, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1982, 46 (05) :1183-1189

[2] A NEW ENZYME NITRILE HYDRATASE WHICH DEGRADES ACETONITRILE IN COMBINATION WITH AMIDASE [J].

ASANO, Y ;

TANI, Y ;

YAMADA, H .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1980, 44 (09) :2251-2252

[3] STEREOSELECTIVE HYDROLYSIS OF NITRILES AND AMIDES UNDER MILD CONDITIONS USING A WHOLE-CELL CATALYST [J].

BEARD, T ;

COHEN, MA ;

PARRATT, JS ;

TURNER, NJ ;

CROSBY, J ;

MOILLIET, J .

TETRAHEDRON-ASYMMETRY, 1993, 4 (06) :1085-1104

[4]

BIANCHI D, 1993, APPL MICROBIOL BIOT, V40, P53

[5] QUANTITATIVE-ANALYSES OF BIOCHEMICAL KINETIC RESOLUTIONS OF ENANTIOMERS [J].

CHEN, CS ;

FUJIMOTO, Y ;

GIRDAUKAS, G ;

SIH, CJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (25) :7294-7299

[6] PRODUCTION OF S-(+)-2-PHENYLPROPIONIC ACID FROM (R,S)-2-PHENYLPROPIONITRILE BY THE COMBINATION OF NITRILE HYDRATASE AND STEREOSELECTIVE AMIDASE IN RHODOCOCCUS-EQUI TG328 [J].

GILLIGAN, T ;

YAMADA, H ;

NAGASAWA, T .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1993, 39 (06) :720-725

[7] ASYMMETRIC HYDROLYSIS OF CHIRAL NITRILES BY RHODOCOCCUS-RHODOCHROUS NCIMB-11216 NITRILASE [J].

GRADLEY, ML ;

KNOWLES, CJ .

BIOTECHNOLOGY LETTERS, 1994, 16 (01) :41-46

[8]

HIGO A, 1978, Patent No. 79144332

[9] MICROBIAL HYDROLYSIS AS A POTENT METHOD FOR THE PREPARATION OF OPTICALLY-ACTIVE NITRILES, AMIDES AND CARBOXYLIC-ACIDS [J].

KAKEYA, H ;

SAKAI, N ;

SUGAI, T ;

OHTA, H .

TETRAHEDRON LETTERS, 1991, 32 (10) :1343-1346

[10] AMIDASE COUPLED WITH LOW-MOLECULAR-MASS NITRILE HYDRATASE FROM RHODOCOCCUS-RHODOCHROUS J1 - SEQUENCING AND EXPRESSION OF THE GENE AND PURIFICATION AND CHARACTERIZATION OF THE GENE-PRODUCT [J].

KOBAYASHI, M ;

KOMEDA, H ;

NAGASAWA, T ;

NISHIYAMA, M ;

HORINOUCHI, S ;

BEPPU, T ;

YAMADA, H ;

SHIMIZU, S .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 217 (01) :327-336