DETERMINATION OF AMINO-ACIDS IN HUMAN PLASMA BY LIQUID-CHROMATOGRAPHY WITH POSTCOLUMN NINHYDRIN DERIVATIZATION USING A HYDROXYAPATITE CARTRIDGE FOR PRECOLUMN DEPROTEINATION

被引:20
作者
IWASE, H
OZAWA, S
IKUTA, M
ONO, I
机构
[1] Ajinomoto Co., Central Research Laboratories, Kawasaki-ku, Kawasaki, 210
来源
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 663卷 / 01期
关键词
D O I
10.1016/0378-4347(94)00439-C
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Amino acids in human plasma were determined by liquid chromatography with postcolumn ninhydrin derivatization using a hydroxyapatite cartridge for precolumn deproteination. S-Carboxymethyl-L-cysteine, D-phenylglycine and S-aminoethyl-L-cysteine were found to be suitable internal standards. The proposed method is simple, rapid (deproteination time less than 1 min) and reproducible [relative standard deviation below 3% except for low-level aspartic acid (n = 3)]. The average recovery of 25 amino acids was above 90%. The elution time of amino acids in human plasma was approximately 2 h. Protein binding of tryptophan was also determined by the proposed method. The analytical data for amino acids in human plasma deproteinated using the proposed and published methods (5-sulphosalicylic acid and ethanol) were compared.
引用
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页码:15 / 24
页数:10
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