CALCIUM-BINDING SITE(S) OF PHOTOSYSTEM-II AS PROBED BY LANTHANIDES

被引:9
作者
BAKOU, A
BUSER, C
DANDULAKIS, G
BRUDVIG, G
GHANOTAKIS, DF
机构
[1] UNIV CRETE,DEPT CHEM,IRAKLION,GREECE
[2] YALE UNIV,DEPT CHEM,NEW HAVEN,CT 06520
关键词
CALCIUM; EPR; OXYGEN EVOLUTION; LANTHANIDE; PHOTOSYSTEM-II;
D O I
10.1016/0167-4838(92)90436-H
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lanthanides and calcium compete for binding sites on the oxidizing side of Photosystem II. When the trivalent ions replace calcium, the oxygen evolving complex is unable to proceed to the higher oxidation states. Oxygen-evolution activity can be reconstituted by removing the lanthanide and adding back calcium. The number of lanthanide atoms bound to PS II preparations with and without the 17 and 23 kDa species is the same. This is taken as evidence that the extrinsic 17 and 23 kDa do not bind lanthanide/calcium ions. A low-temperature EPR study, with both diamagnetic and paramagnetic lanthanide ions, has demonstrated a close interaction between the lanthanides and the tyrosine radical Y(D+).
引用
收藏
页码:131 / 136
页数:6
相关论文
共 25 条
[21]   A MARKED UPSHIFT IN THRESHOLD TEMPERATURE FOR THE S1-TO-S2 TRANSITION INDUCED BY LOW PH TREATMENT OF PS-II MEMBRANES [J].
ONO, TA ;
INOUE, Y .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1015 (02) :373-377
[22]  
RADMER R, 1977, PRIMARY PROCESSES PH, P308
[23]   THE CHLORIDE REQUIREMENT FOR PHOTOSYNTHETIC OXYGEN EVOLUTION - ANALYSIS OF THE EFFECTS OF CHLORIDE AND OTHER ANIONS ON AMINE INHIBITION OF THE OXYGEN-EVOLVING COMPLEX [J].
SANDUSKY, PO ;
YOCUM, CF .
BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 766 (03) :603-611
[24]   A CALCIUM-SPECIFIC SITE INFLUENCES THE STRUCTURE AND ACTIVITY OF THE MANGANESE CLUSTER RESPONSIBLE FOR PHOTOSYNTHETIC WATER OXIDATION [J].
SIVARAJA, M ;
TSO, J ;
DISMUKES, GC .
BIOCHEMISTRY, 1989, 28 (24) :9459-9464
[25]  
TAMURA N, 1988, LIGHT ENERGY TRANSDU, P227