PSEUDOMONAS-AERUGINOSA EXOENZYME-S IS AN ADHESIN

被引：69

作者：

BAKER, NR

MINOR, V

DEAL, C

SHAHRABADI, MS

SIMPSON, DA

WOODS, DE

机构：

[1] OHIO STATE UNIV,DEPT MICROBIOL,COLUMBUS,OH 43210

[2] WALTER REED ARMY MED CTR,DEPT BACTERIAL DIS,WASHINGTON,DC 20307

[3] UNIV CALGARY,HLTH SCI CTR,DEPT MICROBIOL & INFECT DIS,CALGARY T2N 4N1,ALBERTA,CANADA

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 09期

关键词：

D O I：

10.1128/IAI.59.9.2859-2863.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Exoenzyme S from Pseudomonas aeruginosa has been studied as an adhesin for glycosphingolipids and buccal cells. Binding of exoenzyme S to gangliotriosylceramide (GalNAc-beta-1-4Gal-beta-1-4Glc-beta-1-1Cer), gangliotetraosylceramide (Gal-beta-1-3GalNAcT-beta-1-4Gal-beta-1-4Glc-beta-1-1Cer), and lactosylceramide (Gal-beta-1-4Glc-beta-1-1Cer) separated on thin-layer chromatograms was observed. Binding curves for exoenzyme S with dilutions of gangliotetraosylceramide immobilized on plastic plates were similar to previously reported results for the intact bacteria. Binding of exoenzyme S to sialylated counterparts of these glycosphingolipids was not seen, indicating that the addition of a sialic acid residue interferes with binding. Exoenzyme S and monoclonal antibody to exoenzyme S inhibit the binding of P. aeruginosa to buccal cells. The presence of exoenzyme S on the surface of P. aeruginosa was detected by immunogold labeling of bacteria with antibodies to exoenzyme S. Results of these studies led us to conclude that exoenzyme S is an important adhesin of P. aeruginosa.

引用

页码：2859 / 2863

页数：5

共 30 条

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