ISOLATION AND IDENTIFICATION BY GAS-CHROMATOGRAPHIC MASS-SPECTROMETRY OF THE CARBONYL-ACTIVE SITE OF PIG-KIDNEY DIAMINE OXIDASE

An adduct with phenylhydrazine was formed with the purified pig kidney diamine oxidase and in parallel with the I-tyrosine decarboxylase from Streptococcus faecalis. The labeled enzymes were hydrolyzed by chemical hydroIysis and the adducts released by hydrolysis were isolated and identified first in HPLC and successively in GC-MS. Both enzymes gave the same adduct which was identified as the phenylhydrazone of pyridoxal. The isolated adduct had the same retention time as the phenylhydrazone of pyridoxal in HPLC and in gas chromatography and showed the same molecular weight in mass spectrometry when chemical ionization was used and the same fragmentation in mass spectrometry when electronic impact was used. The reported results show that pig kidney diamine oxidase contains pyridoxal in the form of covalently linked pyridoxal phosphate which can be released from the enzyme only by chemical hydrolysis. Pig kidney diamine oxidase is therefore a pyridoxal enzyme such as I-tyrosine decarboxylase as hypothesized in the past but never clearly demonstrated. (c) 1994 Academic Press, Inc.