NEUROTROPHIN-3 UP-REGULATES NGF RECEPTORS IN A CENTRAL-NERVOUS-SYSTEM GLIAL-CELL LINE

被引：12

作者：

SPOERRI, PE ^{[1
]}

ROMANELLO, S ^{[1
]}

PETRELLI, L ^{[1
]}

NEGRO, A ^{[1
]}

GUIDOLIN, D ^{[1
]}

SKAPER, SD ^{[1
]}

机构：

[1] FIDIA RES LABS,FIDIA RES LABS,VIA PONTE FABBRICA 3-A,I-35031 ABANO TERME,ITALY

来源：

NEUROREPORT | 1993年 / 4卷 / 01期

关键词：

NERVE GROWTH FACTOR RECEPTOR; NEUROTROPHIN-3; UP-REGULATION; GLIAL CULTURE; IMMUNOLOCALIZATION; MESSENGER RNA;

D O I：

10.1097/00001756-199301000-00008

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE low-affinity nerve growth factor (NGF) receptor (LNGFR) binds the neurotrophins NGF, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) with similar affinities. Here we report on the ability of NT-3 to regulate the expression of the LNGFR in C6 glioma cells. LNGFR-like immunoreactivity (LNGFR-IR) was examined in C6 cells treated for 16 h with NT-3 and exposed to the antibody 192-IgG followed by immunoglobulins conjugated with colloidal gold by means of ultrastructural morphometric analysis. Untreated C6 cells exhibited some positive LNGFR-IR, while C6 cells treated with NT-3 displayed significantly increased (2.3fold) LNGFR-IR. The increase in LNGFR protein was accompanied by a greater quantity of LNGFR mRNA in NT-3-treated cells. Thus, LNGFR can be upregulated by the structurally related neurotrophin NT-3.

引用

页码：33 / 36

页数：4

共 25 条

[1] TROPHIC FACTORS AND NEURONAL SURVIVAL [J].

BARDE, YA .

NEURON, 1989, 2 (06) :1525-1534

[2] PURIFICATION OF A NEW NEUROTROPHIC FACTOR FROM MAMMALIAN BRAIN [J].

BARDE, YA ;

EDGAR, D ;

THOENEN, H .

EMBO JOURNAL, 1982, 1 (05) :549-553

[3]

BERND P, 1984, J BIOL CHEM, V259, P5509

[4]

CHANDLER CE, 1984, J BIOL CHEM, V259, P6882

[5] GENE-TRANSFER AND MOLECULAR-CLONING OF THE HUMAN NGF RECEPTOR [J].

CHAO, MV ;

BOTHWELL, MA ;

ROSS, AH ;

KOPROWSKI, H ;

LANAHAN, AA ;

BUCK, CR ;

SEHGAL, A .

SCIENCE, 1986, 232 (4749) :518-521

[6] MOLECULAR-CLONING AND NEUROTROPHIC ACTIVITIES OF A PROTEIN WITH STRUCTURAL SIMILARITIES TO NERVE GROWTH-FACTOR - DEVELOPMENTAL AND TOPOGRAPHICAL EXPRESSION IN THE BRAIN [J].

ERNFORS, P ;

IBANEZ, CF ;

EBENDAL, T ;

OLSON, L ;

PERSSON, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (14) :5454-5458

[7]

HALLBROOK F, 1992, NEURON, V89, P3060

[8] EXPRESSION OF FUNCTIONAL NERVE GROWTH-FACTOR RECEPTORS AFTER GENE-TRANSFER [J].

HEMPSTEAD, BL ;

SCHLEIFER, LS ;

CHAO, MV .

SCIENCE, 1989, 243 (4889) :373-375

[9] NGF INDUCTION OF NGF RECEPTOR GENE-EXPRESSION AND CHOLINERGIC NEURONAL HYPERTROPHY WITHIN THE BASAL FOREBRAIN OF THE ADULT-RAT [J].

HIGGINS, GA ;

KOH, SY ;

CHEN, KS ;

GAGE, FH .

NEURON, 1989, 3 (02) :247-256

[10] IDENTIFICATION AND CHARACTERIZATION OF A NOVEL MEMBER OF THE NERVE GROWTH-FACTOR BRAIN-DERIVED NEUROTROPHIC FACTOR FAMILY [J].

HOHN, A ;

LEIBROCK, J ;

BAILEY, K ;

BARDE, YA .

NATURE, 1990, 344 (6264) :339-341

← 1 2 3 →