NEUROTROPHIN-3 UP-REGULATES NGF RECEPTORS IN A CENTRAL-NERVOUS-SYSTEM GLIAL-CELL LINE

被引：12

作者：

SPOERRI, PE ^{[1
]}

ROMANELLO, S ^{[1
]}

PETRELLI, L ^{[1
]}

NEGRO, A ^{[1
]}

GUIDOLIN, D ^{[1
]}

SKAPER, SD ^{[1
]}

机构：

[1] FIDIA RES LABS,FIDIA RES LABS,VIA PONTE FABBRICA 3-A,I-35031 ABANO TERME,ITALY

来源：

NEUROREPORT | 1993年 / 4卷 / 01期

关键词：

NERVE GROWTH FACTOR RECEPTOR; NEUROTROPHIN-3; UP-REGULATION; GLIAL CULTURE; IMMUNOLOCALIZATION; MESSENGER RNA;

D O I：

10.1097/00001756-199301000-00008

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE low-affinity nerve growth factor (NGF) receptor (LNGFR) binds the neurotrophins NGF, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) with similar affinities. Here we report on the ability of NT-3 to regulate the expression of the LNGFR in C6 glioma cells. LNGFR-like immunoreactivity (LNGFR-IR) was examined in C6 cells treated for 16 h with NT-3 and exposed to the antibody 192-IgG followed by immunoglobulins conjugated with colloidal gold by means of ultrastructural morphometric analysis. Untreated C6 cells exhibited some positive LNGFR-IR, while C6 cells treated with NT-3 displayed significantly increased (2.3fold) LNGFR-IR. The increase in LNGFR protein was accompanied by a greater quantity of LNGFR mRNA in NT-3-treated cells. Thus, LNGFR can be upregulated by the structurally related neurotrophin NT-3.

引用

页码：33 / 36

页数：4

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