RELATION BETWEEN ACTIVITY AND ZINC AND CHLORIDE BINDING OF ESCHERICHIA-COLI ALKALINE-PHOSPHATASE

被引：8

作者：

NORNE, JE ^{[1
]}

SZAJN, H ^{[1
]}

CSOPAK, H ^{[1
]}

REIMARSSON, P ^{[1
]}

LINDMAN, B ^{[1
]}

机构：

[1] GOTHENBURG UNIV,INST BIOCHEM,S-40220 GOTHENBURG,SWEDEN

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1979年 / 196卷 / 02期

关键词：

D O I：

10.1016/0003-9861(79)90307-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The relation between Zn2+ binding of E. coli alkaline phosphatase and enzymatic activity and anion binding (using 35Cl NMR) has been investigated. The results suggest the existence of two forms of the enzyme with different zinc binding properties. The anion binding associated with the enzyme's function appears to be an amino acid residue and not the Zn2+ ions; furthermore, there is a rapid internal motion at the anion binding site. 35Cl relaxation studies in the presence of Mg2+ ions point to a marked interdependence of Mg2+ and Zn2+ binding. © 1979.

引用

页码：552 / 556

页数：5

共 32 条

[1] EFFECT OF MG(II) ON SPECTRAL PROPERTIES OF CO(II) ALKALINE-PHOSPHATASE [J].

ANDERSON, RA ;

KENNEDY, FS ;

VALLEE, BL .

BIOCHEMISTRY, 1976, 15 (17) :3710-3716

[2]

APPLEBUR.ML, 1970, J BIOL CHEM, V245, P4968

[3] CD-113 NMR AS A PROBE OF ACTIVE-SITES OF METALLOENZYMES [J].

ARMITAGE, IM ;

UITERKAMP, AIMS ;

CHLEBOWSKI, JF ;

COLEMAN, JE .

JOURNAL OF MAGNETIC RESONANCE, 1978, 29 (02) :375-392

[4] EFFECT OF MAGNESIUM ON PROPERTIES OF ZINC ALKALINE-PHOSPHATASE [J].

BOSRON, WF ;

ANDERSON, RA ;

FALK, MC ;

KENNEDY, FS ;

VALLEE, BL .

BIOCHEMISTRY, 1977, 16 (04) :610-614

[5] NUCLEAR MAGNETIC-RESONANCE STUDIES OF CHLORIDE BINDING TO PROTEINS [J].

BULL, T ;

NORNE, JE ;

REIMARSSON, P ;

LINDMAN, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1978, 100 (15) :4643-4647

[6] CL-35 NUCLEAR MAGNETIC-RELAXATION STUDIES OF CL-BINDING TO LACTATE-DEHYDROGENASE [J].

BULL, TE ;

LINDMAN, B ;

REIMARSSON, P .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1976, 176 (01) :389-391

[7]

CHLEBOWSKI JF, 1977, J BIOL CHEM, V252, P7053

[8]

CHLEBOWSKI JF, 1976, MET IONS BIOL SYST, V6, P1

[9] FACTORS AFFECTING ZINC CONTENT OF ESCHERICHIA-COLI ALKALINE-PHOSPHATASE [J].

CSOPAK, H ;

SZAJN, H .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 157 (02) :374-379

[10] SPECIFIC BINDING OF ZINC(2) TO ALKALINE PHOSPHATASE OF ESCHERICHIA COLI [J].

CSOPAK, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 7 (02) :186-&

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