ATTENUATED MENGO-VIRUS AS A VECTOR FOR IMMUNOGENIC HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GLYCOPROTEIN-120

被引：31

作者：

ALTMEYER, R ^{[1
]}

ESCRIOU, N ^{[1
]}

GIRARD, M ^{[1
]}

PALMENBERG, A ^{[1
]}

VANDERWERF, S ^{[1
]}

机构：

[1] UNIV WISCONSIN,INST MOLEC VIROL,MADISON,WI 53706

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 21期

关键词：

VACCINE;

D O I：

10.1073/pnas.91.21.9775

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Introduction of a sequence encoding 147 amino acids from human immunodefiency virus type I (HIV-1) strain MN glycoprotein gp120 into the RNA genome of the stably attenuated Mengo virus strain vM16 yielded an infectious recombinant virus, vMLN450, which expressed the heterologous HIV-1 sequence along with the normal Mengo virus proteins. The HIV-1 gp120 sequence, fused to the amino terminus of the short, nonstructural Mengo virus leader polypeptide was recognized by a gp120 V3 loop-specific monoclonal antibody. When inoculated into mice, recombinant virus vMLN450 elicited a high-titer anti-HIV-1 antibody response as well as an HIV-1(MN)-specific cytotoxic cellular immune response. An anti-HIV-1 antibody response could also be detected in cynomolgus monkeys after a single immunization. We propose that attenuated Mengo virus can serve as an effective expression vector in cell systems and various animal species and offers another approach to the development of new, live recombinant vaccines.

引用

页码：9775 / 9779

页数：5

共 24 条

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