DOWN-REGULATION OF VASOPRESSIN V1A RECEPTOR MESSENGER-RNA IN DIABETES-MELLITUS IN THE RAT

被引：16

作者：

PHILLIPS, PA ^{[1
]}

RISVANIS, J ^{[1
]}

HUTCHINS, AM ^{[1
]}

BURRELL, LM ^{[1
]}

MACGREGOR, D ^{[1
]}

GUNDLACH, AL ^{[1
]}

JOHNSTON, CI ^{[1
]}

机构：

[1] UNIV MELBOURNE,AUSTIN HOSP,CLIN PHARMACOL & THERAPEUT UNIT,HEIDELBERG,VIC 3084,AUSTRALIA

来源：

CLINICAL SCIENCE | 1995年 / 88卷 / 06期

关键词：

ANTIDIURETIC HORMONE; HEPATOCYTE; MESSENGER-RNA; RECEPTORS; VASOPRESSIN;

D O I：

10.1042/cs0880671

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

1.To investigate the mechanism of hepatic Via vasopressin receptor down-regulation in streptozotocin-induced diabetes mellitus in the rat, we measured hepatic V1a receptor mRNA by in situ hybridization histochemistry using oligonucleotide probes to the V1a receptor and Northern blotting. 2. Diabetes mellitus caused hyperglycaemia, hyperosmolality and increased plasma vasopressin concentrations (P<0,01), Hepatoycte Via receptor mRNA was reduced by 76% in diabetic rats (P< 0.01) and by 53% in insulin-treated diabetic rats (P<0,01) versus control rats, in parallel with reduced Via radioligand binding and vasopressin-stimulated inositol phosphates production, There was a similar decrease in hepatic V1a/18S mRNA density ratio in the diabetic and diabetic+insulin groups (both P<0,05 versus control). 3. These findings suggest that altered V1a mRNA transcription is responsible for the reduced hepatic V1a receptor density in diabetes mellitus.

引用

页码：671 / 674

页数：4