CONTRIBUTIONS TO THE S'-SUBSITE SPECIFICITY OF PAPAIN

被引:21
作者
SCHUSTER, M
KASCHE, V
JAKUBKE, HD
机构
[1] KARL MARX UNIV LEIPZIG,DEPT BIOCHEM,DIV BIOSCI,TALSTR 33,O-7010 LEIPZIG,GERMANY
[2] TECH UNIV HAMBURG,DEPT BIOTECHNOL,HAMBURG,GERMANY
关键词
PAPAIN; ACYL TRANSFER; SUBSITE SPECIFICITY;
D O I
10.1016/0167-4838(92)90356-I
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The product ratio was analyzed for the papain-catalyzed acyl transfer from the specific acyl donor Mal-Phe-Ala-OEtCl to various nucleophilic amino components, ranging from amino acid amides to tripeptide amides. The data obtained are discussed in terms of binding specificity. From the structure-activity relationships for the S1'-P1' interaction it follows that only three methyl(ene) groups can be accommodated in the S1' subsite. Hydrophilic side chains are bound better to S1' than indicated by their hydrophobicities. Negatively charged amino components are inefficient deacylating agents. However, there was no evidence for electrostatic contributions to the nucleophile binding. Amino components with bulky hydrophobic amino acid residues in the P2' and in the P3' position, respectively, are preferentially bound to Mal-Phe-Ala-papain. The results of this study can be applied to the planning of papain-catalyzed peptide synthesis reactions.
引用
收藏
页码:207 / 212
页数:6
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