EXPRESSION OF BOVINE ADRENODOXIN IN ESCHERICHIA-COLI AND SITE-DIRECTED MUTAGENESIS OF (2-FE-2S) CLUSTER LIGANDS

被引：107

作者：

UHLMANN, H ^{[1
]}

BECKERT, V ^{[1
]}

SCHWARZ, D ^{[1
]}

BERNHARDT, R ^{[1
]}

机构：

[1] MAX DELBRUCK CTR MOLEC MED, ROBERT ROSSLE STR 10, O-1115 BERLIN, GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 188卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91349-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression systems for adrenodoxin into the periplasm and the cytoplasm of E. coli have been developed as a prerequisite for site-directed mutagenesis studies. In both systems the /2Fe2S/ cluster of the protein was correctly assembled, the cytoplasmic one gives, however, a tenfold higher expression level. To determine which of the five cysteines at positions 46, 52, 55, 92, and 95 coordinate the /2Fe2S/ center, they have been individually mutated into serines. From these mutants, only C95S forms a functionally active holoprotein. Thus, residues 46, 52, 55, and 92 are the cysteines that coordinate the /2Fe2S/ cluster in adrenodoxin. © 1992.

引用

页码：1131 / 1138

页数：8

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