THE EXPRESSION OF A SPECIFIC 2-DEOXYGLUCOSE-6P PHOSPHATASE PREVENTS CATABOLITE REPRESSION MEDIATED BY 2-DEOXYGLUCOSE IN YEAST

2-deoxyglucose (2-DOG), a non-metabolize analogue of glucose, is taken up by yeast using the same transporter(s) as glucose and is phosphorylated by hexokinases producing 2-deoxyglucose-6-P. We found that in DOG(R) yeasts, 2-DOG was not able to trigger glucose repression, even at concentrations of 0.5%. This result suggests that the specific 2-DOG-6P phosphatase, the enzyme responsible for the DOG(R) phenotype, may be involved in inhibiting the process of catabolite repression mediated by 2-DOG.