DETECTION AND CHARACTERIZATION OF DNA-POLYMERASE FROM TRYPANOSOMA-BRUCEI

被引：21

作者：

DUBE, DK ^{[1
]}

WILLIAMS, RO ^{[1
]}

SEAL, G ^{[1
]}

WILLIAMS, SC ^{[1
]}

机构：

[1] INT LAB RES ANIM DIS,NAIROBI,KENYA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 561卷 / 01期

基金：

美国国家卫生研究院;

关键词：

(Trypanosoma brucei); DNA polymerase; Kinetoplastida; Parasitic protozoa;

D O I：

10.1016/0005-2787(79)90485-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The predominant DNA polymerase activity has been isolated from the parasitic flagellated protozoan, Trypanosoma brucei. Like mammalian DNA polymerase-α the trypanosome DNA polymerase is of large molecular weight (S, 6-8), is resistant to thermal denaturation, is sensitive to N-ethylmaleimide, and is inhibited by high ionic strength. However, specific antisera that crossreact with mammalian DNA polymerase-α from different species fail to crossreact with the trypanosome polymerase. © 1979.

引用

页码：10 / 16

页数：7

共 21 条

[11]

JOVIN TM, 1969, J BIOL CHEM, V244, P2996

[12] PURIFICATION OF DNA POLYMERASE OF AVIAN MYELOBLASTOSIS VIRUS [J].