MICROBIAL-FLORA IN SEMEN DURING INVITRO FERTILIZATION

被引：23

作者：

HUYSER, C

FOURIE, FL

OOSTHUIZEN, M

NEETHLING, A

机构：

[1] Reproductive Biology Research Unit, Department of Obstetrics, and Gynaecology, University of Pretoria, Pretoria, 0002

[2] Department of Microbiology, University of Pretoria, Pretoria

[3] Institute for Biostatistics, Medical Research Council, Pretoria

来源：

JOURNAL OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER | 1991年 / 8卷 / 05期

关键词：

INVITRO FERTILIZATION; SPERM SEMEN BACTERIOLOGY; ANTIBIOTIC TREATMENT;

D O I：

10.1007/BF01139781

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Semen samples from 183 consecutive unselected men participating in an in vitro fertilization program were retrospectively studied to determine the bacterial and fungal contamination rate before and after antibiotic treatment. To ascertain the influence of semen preparation (wash and swim-up method) on the incidence of microorganisms, semen from 102 male patients was studied before and after swimup. Antimicrobial treatment by prescription of antibiotics decreased the incidence of pathogens by 16.3% (P < 0.0001). Semen processing was more effective by ridding 57.4% of semen samples of microbial contaminants (P < 0.0001). When infection of culture media was observed during routine microscopy, all infected oocytes were degenerated, without evidence of fertilization or pronuclei.

引用

页码：260 / 264

页数：5