PREPARATION AND CRYSTALLIZATION OF D-GLUCOSAMINE OLIGOSACCHARIDES WITH DP 6-8

被引：48

作者：

MURAKI, E

YAKU, F

KOJIMA, H

机构：

[1] Government Industrial Research Osaka, Ikeda-shi, Osaka, 563

来源：

CARBOHYDRATE RESEARCH | 1993年 / 239卷

关键词：

D O I：

10.1016/0008-6215(93)84217-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

D-Glucosamine oligosaccharides of degree of polymerization 5-9 were separated from a chitosan hydrolyzate prepared with the cellulase of Trichoderma viride. Separation was accomplished by prefractionation with methanol-water and chromatography on a weak acid ion-exchanger, with elution by 0.01 N HCI. Penta- to nona-saccharides comprised almost half of the the total product. The hexa-, hepta-, and octa-saccharides were crystallized from dilute HCI solutions as their hydrochloride salts. Conductometric titration to determine the glucosamine content and elemental analysis showed that this procedure is effective for the preparation of highly pure oligosaccharides.

引用

页码：227 / 237

页数：11

共 15 条

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