EVANESCENT-WAVE IMMUNOPROBE WITH HIGH BIVALENT ANTIBODY-ACTIVITY

被引：11

作者：

FELDMAN, SF

UZGIRIS, EE

PENNEY, CM

GUI, JY

SHU, EY

STOKES, EB

机构：

[1] General Electric Corporate Research and Development, Schenectady

来源：

BIOSENSORS & BIOELECTRONICS | 1995年 / 10卷 / 05期

关键词：

EVANESCENT WAVE FIBER IMMUNOSENSOR; ANTIBODY ANTIGEN KINETICS; UNSTIRRED DEAD LAYER THICKNESS; IMMUNOSENSOR RESPONSE; OPTICAL FIBER SENSOR;

D O I：

10.1016/0956-5663(95)96889-7

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have determined the kinetic response of an evanescent wave optical fibre immunosensor and its absolute sensitivity. Using both kinetic methods and optical determinations of bound antigen, we infer that there are 2.4 x 10(11) active antibodies per cm(2) probe area. We estimate that 75% of the active antibodies are in the bivalent form, with both binding sites capable of binding antigen. Using the kinetic data and optical measurements of the number of antigens bound to the fibre, we estimate that the unstirred dead layer thickness next to the fibre surface in the stirred solution, across which the antigens must diffuse, is 55 mu m. Approximately 10(9) FITC labelled antigens are necessary to achieve a threshold signal, defined as 1% of the signal obtained when the fibre is saturated with antigen.

引用

页码：423 / 434

页数：12

共 23 条

[1]

Aizenbud, Volterra, Priel, Kinetic model for the irreversible adsorption of macromolecules at a liquid-solid interface, J. Colloid & Interface Sci., 103, pp. 133-138, (1985)

[2]

Anderson, Darst, Robertson, Protein adsorption at polymer surfaces: A study using total internal reflection fluorescence, Proteins at Interfaces: Physiocochemical & Biochemical Studies, pp. 306-323, (1987)

[3]

Berg, Purcell, Physics of chemoreception, Biophysics J., 20, pp. 193-219, (1977)

[4]

Bhatia, Shriver-Lake, Prior, Georger, Calvert, Bredehorst, Ligler, Use of thiol-terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces, Anal. Biochem., 178, pp. 408-413, (1989)

[5]

Bhatia, Thompson, Shriver-Lake, Levine, Ligler, Fiber optic-based immunosensors A progress report, Flourescence Detection III, SPIE, 1054, pp. 184-190, (1989)

[6]

Bull, An Introduction to Physical Biochemistry, (1971)

[7]

Corsel, Willems, Kop, Cuypers, Hermens, The role of intrinsic binding rate and transport rate in the adsorption of prothrombin, albumin, and fibrinogen to phospholipid bilayers, J. Colloidal & Interface Sci., 111, pp. 544-554, (1986)

[8]

Feder, Giaever, Adsorption of Ferritin, J. Colloidal & Interface Sci., 78, pp. 144-154, (1980)

[9]

Feldman, Uzgiris, Determination of the kinetic response and absolute sensitivity of a fiber-optic immunoassay, Chem. Biochem. & Environ. Fiber Sensors V, SPIE, 2068, pp. 139-144, (1993)

[10]

Feldman, Shu, Gui, Synthesis of tapers for fiber optic sensors, US Patent #5,290,398, (1994)

← 1 2 3 →