PURIFICATION AND CHARACTERIZATION OF MANNITOL DEHYDROGENASE FROM THE FUNGAL TOMATO PATHOGEN CLADOSPORIUM-FULVUM (SYN FULVIA-FULVA)

被引：19

作者：

NOELDNER, PKM

COLEMAN, MJ

FAULKS, R

OLIVER, RP

机构：

[1] UNIV E ANGLIA,SCH BIOL SCI,NORWICH MOLEC PLANT PATHOL GRP,NORWICH NR4 7TJ,NORFOLK,ENGLAND

[2] NORWICH LAB,INST FOOD RES,NORWICH,NORFOLK,ENGLAND

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 1994年 / 45卷 / 04期

关键词：

D O I：

10.1016/S0885-5765(05)80059-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

As part of our studies to elucidate the mechanisms by which biotrophic plant pathogensobtain and retain carbon from their plant hosts, we have investigated the properties of the mannitol dehydrogenase (E.C.1.1.1.67) of Cladosporium fulvum (syn. Fulvia fulva). The enzyme has been purified to near-homogeneity and has a subunit mol. wt of 29 kDa and is probably tetrameric. The Michaelis-Menten constants for mannitol and fructose are high and the equilibrium constant lies towards mannitol accumulation. A beta-fructosidase (invertase) activity was detected and found to be largely wall-bound. The properties of these enzymes are consistent with a role in the hydrolysis of photosynthetically produced sucrose and the accumulation of mannitol as a strong compound during fungal growth in the plant.

引用

页码：281 / 289

页数：9

共 15 条

[11] DISRUPTION OF THE AVIRULENCE GENE AVR9 IN 2 RACES OF THE TOMATO PATHOGEN CLADOSPORIUM-FULVUM CAUSES VIRULENCE ON TOMATO GENOTYPES WITH THE COMPLEMENTARY RESISTANCE GENE CF9 [J].