ANALYSIS OF ODOR-ACTIVE VOLATILES FROM PSEUDOMONAS-FRAGI GROWN IN MILK

Pseudomonas fragi produced a pleasant strawberry-like odor when grown in skim milk at 15-degrees-C. Volatiles from the culture broth were extracted and enriched by using a purge and trap method and analyzed by using sniffing FID-GC. FID revealed the presence of approximately 90 compounds. Concomitant olfactory analysis of the eluted compounds demonstrated that 26 were odor-active. Ethyl butyrate, ethyl 3-methylbutanoate, and ethyl hexanoate were the major contributors to the odor, while other compounds contributed to its complexity and richness.