A NOVEL, TISSUE-RESTRICTED ZINC-FINGER PROTEIN (HF-1B) BINDS TO THE CARDIAC REGULATORY ELEMENT (HF-1B/MEF-2) IN THE RAT MYOSIN LIGHT-CHAIN 2 GENE

被引:78
作者
ZHU, H
NGUYEN, VTB
BROWN, AB
POURHOSSEINI, A
GARCIA, AV
VANBILSEN, M
CHIEN, KR
机构
[1] UNIV CALIF SAN DIEGO,SCH MED,DEPT MED,CTR MOLEC GENET,LA JOLLA,CA 92093
[2] UNIV CALIF SAN DIEGO,SCH MED,AMER HEART ASSOC BUGHER FDN CTR MOLEC BIOL,LA JOLLA,CA 92093
关键词
D O I
10.1128/MCB.13.7.4432
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The AT-rich element MEF-2 plays an important role in the maintenance of the muscle-specific expression of a number of cardiac and skeletal muscle genes. In the MLC-2 gene, an AT-rich element (HF-1b) which contains a consensus MEF-2 site is required for cardiac tissue-specific expression. The present study reports the isolation and characterization of a cDNA which encodes a novel C2H2 zinc finger (HF-1b) that binds in a sequence-specific manner to the HF-1b/MEF-2 site in the MLC-2 promoter. A number of independent criteria suggest that this HF-1b zinc finger protein is a component of the endogenous HF-1b/MEF-2 binding activity in cardiac muscle cells and that it can serve as a transcriptional activator of the MLC-2 promoter in transient assays. These studies suggest that, in addition to the previously reported RSRF proteins, structurally divergent transcriptional factors can bind to MEF-2-like sites in muscle promoters. These results underscore the complexity of the regulation of the muscle gene program via these AT-rich elements in cardiac and skeletal muscle.
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页码:4432 / 4444
页数:13
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