SPECIATION OF SELENOAMINO ACIDS BY ONLINE HPLC ETAA SPECTROMETRY

被引：39

作者：

POTINGAUTIER, M

BOUCHARAT, C

ASTRUC, A

ASTRUC, M

机构：

来源：

APPLIED ORGANOMETALLIC CHEMISTRY | 1993年 / 7卷 / 07期

关键词：

SELENOAMINO ACIDS; HPLC ETAAS; SPECIATION; ENVIRONMENT; DETERMINATION;

D O I：

10.1002/aoc.590070720

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An analytical method was developed to determine selenoamino acids in the presence of other compounds. Separation has been achieved by High Performance Liquid Chromatography (HPLC) using electrothermal atomic absorption (ETAA) spectrometry as a very sensitive and element-specific detector. On-line HPLC ETAAS speciation of selenocystine and selenomethionine has been studied, using a laboratory made interface. Analytical characterization of the method has been realized with standard solutions. Using a 100 mul sample loop, the detection limits were calculated as 8 mug l-1 for selenomethionine and 10 mug l-1 for selenocystine with repeatability and reproducibility of 4% and 7% respectively. The method has been applied to the determination of selenoamino acids in an extract of white clover (CRM402) certified for total selenium.

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页码：593 / 598

页数：6

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