A NEW METHOD FOR DIRECT-DETECTION OF LISTERIA-MONOCYTOGENES FROM FOODS BY PCR

被引：47

作者：

MAKINO, SI

OKADA, Y

MARUYAMA, T

机构：

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 10期

关键词：

D O I：

10.1128/AEM.61.10.3745-3747.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The preparation of DNA by alcohol precipitation in the presence of NaI was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat.

引用

页码：3745 / 3747

页数：3

共 8 条

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