RECOMBINANT VACCINIA VIRUSES EXPRESSING GP46 M-2 PROTECT AGAINST LEISHMANIA INFECTION

被引：90

作者：

MCMAHONPRATT, D ^{[1
]}

RODRIGUEZ, D ^{[1
]}

RODRIGUEZ, JR ^{[1
]}

YA, Z ^{[1
]}

MANSON, K ^{[1
]}

BERGMAN, C ^{[1
]}

RIVAS, L ^{[1
]}

RODRIGUEZ, JF ^{[1
]}

LOHMAN, KL ^{[1
]}

RUDDLE, NH ^{[1
]}

ESTEBAN, M ^{[1
]}

机构：

[1] SUNY HLTH SCI CTR, BROOKLYN, NY 11203 USA

来源：

INFECTION AND IMMUNITY | 1993年 / 61卷 / 08期

关键词：

D O I：

10.1128/IAI.61.8.3351-3359.1993

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Leishmania is a genus of parasitic protozoa capable of causing a spectrum of human diseases. The GP46/M-2 membrane glycoprotein has been demonstrated in a murine model system to elicit a protective immune response against infection with Leishmania amazonensis; in highly susceptible BALB/c mice, immunization leads to significant protection against infection. In the present study, for induction of long-term immunological effects, two recombinant vaccinia viruses, derived from the wild type and attenuated variant 48-7 and expressing the GP46/M-2 protein, were constructed; to ensure safety, we used the attenuated vaccinia virus mutant (48-7) as a live vector. Susceptible BALB/c mice immunized with either GP46/M-2-recombinant vaccinia virus were significantly protected against infection with L. amazonensis; 45 to 76% of the animals were completely protected (sterile) against a challenge inoculum of 103 infective organisms. The protectively immunized animals demonstrated T- and B-cell-dependent immunological responses; both lymphokine responses as well as antibody responses and long-term memory are indicative of T-cell activation. This first report of the use of a recombinant vaccinia virus to induce protection against a Leishmania infection indicates that recombinant vaccinia viruses should be of value in the design of a safe and effective vaccine against this parasitic disease.

引用

页码：3351 / 3359

页数：9

共 52 条

[21] IMMUNOPROTECTIVE LEISHMANIA-MAJOR SYNTHETIC T-CELL EPITOPES [J].