BIOSENSORS BASED ON POLYMER NETWORKS FORMED BY GAMMA-IRRADIATION CROSS-LINKING

被引：11

作者：

HEINEMAN, WR

机构：

[1] Deparment of Chemistry, University of Cincinnati, Cincinnati, 45221-0172, OH

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1993年 / 41卷 / 1-2期

关键词：

BIOSENSOR; POLYMER NETWORK; GAMMA IRRADIATION CROSS-LINKING; POLY(N-VINYLPYRROLIDONE); POLY(VINYL ALCOHOL); POLY(DIMETHYLDIALLYLAMMONIUM CHLORIDE); LACTATE OXIDASE; OXYGEN SENSOR; LACTATE SENSOR; NEUROTRANSMITTER SENSOR; MICROELECTRODE; SCREEN PRINTING; SOLID-STATE VOLTAMMETRY;

D O I：

10.1007/BF02918535

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Water-soluble polymers immobilized by gamma radiation have been investigated as a means of developing electrochemical sensors. Enzyme-based sensors for glucose and lactate have been made by immobilizing glucose oxidase and lactate oxidase, respectively, on platinized graphite electrodes. The enzyme is entrapped in a polymeric network of poly(vinyl alcohol) that is formed by gamma radiation crosslinking. Electrodes coated with poly (N-vinylpyrrolidone) and its corresponding monomer and then crosslinked with gamma radiation show an extraction of catecholamines into the polymer film that enhances the analytical signal for their detection by electrochemical oxidation. Poly(dimethyldiallylammonium chloride) spin-coated on a screen-printed electrochemical cell provides sufficient ionic conductivity for the cell to function as a gas sensor for oxygen, which is detected by reduction at a platinum working electrode.

引用

页码：87 / 97

页数：11

共 13 条

[1] CHARACTERIZATION AND SELECTIVITY OF ELECTRODES COATED WITH GAMMA-RADIATION-IMMOBILIZED HYDROGEL MATRICES [J].