CHANGES IN PROTEIN-KINASE-C ISOENZYME EXPRESSION ASSOCIATED WITH APOPTOSIS IN U937 MYELOMONOCYTIC CELLS

被引：56

作者：

PONGRACZ, J ^{[1
]}

TUFFLEY, W ^{[1
]}

JOHNSON, GD ^{[1
]}

DEACON, EM ^{[1
]}

BURNETT, D ^{[1
]}

STOCKLEY, RA ^{[1
]}

LORD, JM ^{[1
]}

机构：

[1] GEN HOSP, LUNG IMMUNOBIOCHEM RES LAB, BIRMINGHAM B4 6NH, W MIDLANDS, ENGLAND

来源：

EXPERIMENTAL CELL RESEARCH | 1995年 / 218卷 / 02期

关键词：

D O I：

10.1006/excr.1995.1176

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Spontaneously apoptotic U937 cells from exponentially growing cell cultures were enriched on discontinuous Percoll density gradients. Increased PKC-beta and reduced PKC-zeta expression were detected in apoptotic cells by Western blotting. Using confocal microscopy, changes in the level of PKC isoenzymes were confirmed and in addition alterations in the subcellular location of PKC isoenzymes were detected in apoptotic cells compared with nonapoptotic cells. The data indicate that the expression of specific PKC isoenzymes is modulated during apoptosis and that PRC-beta and PKC-zeta may play specific roles in the regulation of the apoptotic program. (C) 1995 Academic Press, Inc.

引用

页码：430 / 438

页数：9

共 36 条

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