NONRECIPROCAL REGULATION OF RHODOBACTER-CAPSULATUS AND RHODOBACTER-SPHAEROIDES RECA GENES EXPRESSION

被引：6

作者：

DEHENESTROSA, ARF ^{[1
]}

RIVERA, E ^{[1
]}

BARBE, J ^{[1
]}

机构：

[1] AUTONOMOUS UNIV BARCELONA,FAC SCI,DEPT GENET & MICROBIOL,E-08193 BARCELONA,SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 129卷 / 2-3期

关键词：

RECA GENE INDUCTION; PHOTOTROPHIC BACTERIA; RECA MUTANT; RHODOBACTER CAPSULATUS; RHODOBACTER SPHAEROIDES;

D O I：

10.1016/0378-1097(95)00154-W

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Rhodobacter capsulatus recA gene has been isolated and sequenced. Its deduced amino acid sequence showed the closest identity with the Rhodobacter sphaeroides RecA protein (91% identity). However, the promoter regions of both R. capsulatus and R. sphaeroides recA genes are only 64% similar. An Escherichia coli-like LexA binding site was not present in the upstream region of the R. capsulatus recA gene. Nevertheless, the R. capsulatus recA gene is inducible by DNA damage in both hetero- and phototrophically growing conditions. The R. capsulatus recA gene is poorly induced when inserted into the chromosome of R. sphaeroides, indicating that the recA gene of both bacteria possess different control sequences despite their phylogenetically close relationship.

引用

页码：175 / 181

页数：7

共 13 条

[1] EXPRESSION OF THE META-CLEAVAGE PATHWAY OPERON OF THE TOL PLASMID OF PSEUDOMONAS-PUTIDA IN THE PHOTOTROPHIC BACTERIUM RHODOBACTER-SPHAEROIDES [J].

CALERO, S ;

GARI, E ;

GIBERT, I ;

BARBE, J .

JOURNAL OF BIOTECHNOLOGY, 1989, 12 (3-4) :231-246

[2] MOLECULAR-CLONING, SEQUENCE AND REGULATION OF EXPRESSION OF THE RECA GENE OF THE PHOTOTROPHIC BACTERIUM RHODOBACTER-SPHAEROIDES [J].

CALERO, S ;

DEHENESTROSA, ARF ;

BARBE, J .

MOLECULAR & GENERAL GENETICS, 1994, 242 (01) :116-120

[3] CLONING AND CHARACTERIZATION OF DNA DAMAGE-INDUCIBLE PROMOTER REGIONS FROM BACILLUS-SUBTILIS [J].

CHEO, DL ;

BAYLES, KW ;

YASBIN, RE .

JOURNAL OF BACTERIOLOGY, 1991, 173 (05) :1696-1703

[4] EXPRESSION OF THE RECA GENE OF ESCHERICHIA-COLI IN SEVERAL SPECIES OF GRAM-NEGATIVE BACTERIA [J].

DEHENESTROSA, ARF ;

CALERO, S ;

BARBE, J .

MOLECULAR & GENERAL GENETICS, 1991, 226 (03) :503-506

[5] AUTOREGULATION AND KINETICS OF INDUCTION OF THE RHIZOBIUM-PHASEOLI RECA GENE [J].

DEHENESTROSA, ARF ;

BARBE, J .

MUTATION RESEARCH, 1994, 308 (01) :99-107

[6] MINI-TN5 TRANSPOSON DERIVATIVES FOR INSERTION MUTAGENESIS, PROMOTER PROBING, AND CHROMOSOMAL INSERTION OF CLONED DNA IN GRAM-NEGATIVE EUBACTERIA [J].

DELORENZO, V ;

HERRERO, M ;

JAKUBZIK, U ;

TIMMIS, KN .

JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6568-6572

[7] MECHANISM OF SPECIFIC LEXA CLEAVAGE - AUTODIGESTION AND THE ROLE OF RECA COPROTEASE [J].

LITTLE, JW .

BIOCHIMIE, 1991, 73 (04) :411-422

[8]

MILLER RV, 1990, ANNU REV MICROBIOL, V44, P365

[9] IDENTIFICATION OF DINR, A DNA DAMAGE-INDUCIBLE REGULATOR GENE OF BACILLUS-SUBTILIS [J].

RAYMONDDENISE, A ;

GUILLEN, N .

JOURNAL OF BACTERIOLOGY, 1991, 173 (22) :7084-7091

[10] SEQUENCE OF THE PROVIDENCIA-RETTGERI LEXA GENE AND ITS CONTROL REGION [J].

RIERA, J ;

BARBE, J .

NUCLEIC ACIDS RESEARCH, 1993, 21 (09) :2256-2256

← 1 2 →