REGULATION OF MOVEMENT SPEED BY INTRACELLULAR PH DURING DICTYOSTELIUM-DISCOIDEUM CHEMOTAXIS

被引：37

作者：

VANDUIJN, B

INOUYE, K

机构：

[1] KYOTO UNIV,FAC SCI,DEPT BOT,KYOTO 606,JAPAN

[2] LEIDEN UNIV,ZOOL LABS,CELL BIOL & GENET UNIT,2311 GP LEIDEN,NETHERLANDS

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 11期

关键词：

AMEBOID MOVEMENT; RANDOM MOVEMENT; CAMP; SIGNAL TRANSDUCTION; PH CHANGE;

D O I：

10.1073/pnas.88.11.4951

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Evidence is presented that the chemoattractant-induced cytoplasmic alkalinization in the cellular slime mold Dictyostelium discoideum is essential in regulating locomotion speed during chemotaxis. Intracellular pH was manipulated with weak bases, weak acids, and proton-pump inhibition. Speed of locomotion of individual cells was measured during random and chemotactic movement. We found that (i) an increase of cytoplasmic pH increases the speed of randomly moving cells and (ii) the chemoattractant-induced rise in intracellular pH is essential for the increase in directed locomotion speed upon chemotactic stimulation. In addition, our experiments support the hypothesis that ammonia plays a key role in the thermo- and phototaxis of migrating slugs by increasing the locomotion speed of individual cells through changes in intracellular pH.

引用

页码：4951 / 4955

页数：5

共 28 条

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